Autoimmune And Inflammatory Disease Models

In Vitro and Ex Vivo Models for Immune Research

Autoimmune and inflammatory diseases involve complex interactions among immune cells, inflammatory mediators, signaling pathways, and affected tissues. Disease-relevant in vitro and ex vivo models can help characterize these mechanisms and evaluate therapeutic activity in biologically relevant systems.

WuXi Biology and Testing provides phenotypic and functional cell-based assays across primary cells and established cell lines, complemented by molecular and cellular readouts, as well as ex vivo tissue, pathology, and biomarker assessment. Whether you are evaluating targeted immunomodulatory mechanisms or screening candidate molecules, our customizable workflows generate reliable data to support target validation, mechanism-of-action studies, compound screening, and advancing your preclinical programs.

In Vitro Cell-based Phenotypic Assays

Phenotypic and functional cell-based assays enable the evaluation of immune cell activation, inflammatory responses, and compound activity in disease-relevant cellular systems. Assays can be developed and applied for compound screening, pharmacological characterization, and mechanism-of-action studies.

We offer various phenotypic and functional cell-based assays for assay development and compound screening. Assays are performed using a range of primary cells and established cell lines, with technologies, including ELISA, MSD multiplex, flow cytometry, transwell migration, real-time qPCR and Western blot.

Primary cells:

PBMCs
Eosinophils
Neutrophils
Monocytes
B cells
T cells

Cell lines:

THP1
Jurkat
Raw264.7

Inflammatory Pathway and Cytokine Assays

Inflammatory signaling and cytokine production are central to many autoimmune and inflammatory diseases. Cell-based assays can characterize inflammatory responses following immune stimulation and evaluate how therapeutic candidates modulate cytokine and chemokine production and downstream inflammatory pathways.

Capabilities include:

Immune cell activation and stimulation assays
Pro-inflammatory cytokine and chemokine analysis
Multiplex cytokine profiling by MSD, CBA, Luminex, Olink, etc.
Gene expression analysis by RT-qPCR, NanoString, etc.
Protein expression and signaling analysis
Compound dose-response and inhibition studies

Ex Vivo Tissue and Pathology Analysis

Ex vivo tissue analysis complements cell-based assays by enabling characterization of inflammatory responses, immune-cell infiltration, tissue pathology, and biomarker expression within the tissue microenvironment. Integrated pathology and tissue-based analytical platforms support the evaluation of disease biology and pharmacodynamic responses across autoimmune and inflammatory research.

Capabilities include: