Assay Development

Biochemical Assay Development for Drug Discovery

WuXi Biology and Testing develops and optimizes biochemical assays using a broad range of formats and technologies to support target-based drug discovery. Our teams tailor assay conditions and readouts to the target, mechanism, and intended screening application.

From assay selection and development through optimization and validation, our biochemical assay capabilities deliver reliable, quantitative insights to support hit identification, hit-to-lead, lead optimization, selectivity profiling, and mechanism-of-action studies. Assays can also be adapted and validated for high-throughput screening (HTS), enabling efficient evaluation of compounds at scale.

Biochemical Assay Development and Validation

We offer comprehensive biochemical assay development and validation services across major therapeutic target classes, including kinases, proteases, deubiquitinases, phosphatases, hydrolases, transferases, transcription factors, cytokines, integrins, E3 ligases, GPCRs and other membrane proteins, nuclear receptors, and nucleic-acid-binding proteins.

Our experienced teams leverage diverse biochemical assay formats and technologies to develop and optimize robust, reproducible assays validated with reference compounds. These assays support drug discovery through reliable target characterization, compound evaluation, and translational insights, and are designed to be compatible with high-throughput screening (HTS) when required.

Instrument and Technology Platforms

Our integrated instrumentation and automation platforms support biochemical assay development, optimization, and plate-based screening across a range of detection and analytical formats, such as TR-FRET, FP, AlphaLISA, ELISA, etc.